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Description
Human / Porcine Insulin ELISA KitProduct Specification Synonym IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin Description Testing Principle This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti Human Porcine Insulin antibody is pre coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme labeled plate. After incubation, the Human Porcine Insulin present in the samples combines
Product Specification
| Synonym | IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin |
| Description |
Testing Principle This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti- Human / Porcine Insulin antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the Human / Porcine Insulin present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).
Detection type: sandwich enzyme immunoassay Form: pre-coated 96-well plate Sample type: Cell culture supernatant, Serum, Plasma Sample quantity: 100ul Kit composition: 96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification Sensitivity: 2.15 pmol / mL Detection range: 15.6 - 1000 pmol/mL Recovery rate: 85.0-108.9% Storage: 2-8 ° C The standard curve: Background: Insulin Insulin and IGF are members of the insulin molecule family. IGF-I and II are structurally homologous to proinsulin and can be thought of as an exaggerated inverted G shape. The G part of the chain is inchain disulfide bonded. Single-stranded proinsulin peptide is composed of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589) and 21 amino acid residues of A chain (aa 90110).The removal of C peptide by proteolysis results in the formation of mature insulin, which is the heterodimers formed by disulfide bonds between A and B chains. Circulating C peptide levels are elevated in hyperinsulinemia, obesity and type 2 diabetes.
Technical hints: For research use only, not for in vitro diagnosis. |
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