SKU: 73263750516

CD98/SLC3A2 His Tag Protein, Human

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Description

CD98/SLC3A2 His Tag Protein, HumanProduct Specification Species Human Synonyms CD98, SLC3A2, 4F2, 4F2HC, 4T2HC, CD98HC, MDU1, NACAE Accession P08195 Amino Acid Sequence Arg206 Ala630, with N terminal 8*His

Product Specification


Species Human
Synonyms CD98, SLC3A2, 4F2, 4F2HC, 4T2HC, CD98HC, MDU1, NACAE
Accession P08195
Amino Acid Sequence Arg206-Ala630, with N-terminal 8*His HHHHHHHHGGGSRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA
Expression System HEK293
Molecular Weight 55-72kDa
Purity >95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage 12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Reference

1、Fenczik C A. et al. (2001) Distinct domains of CD98hc regulateintegrins and amino acid transport. J Biol Chem. 276(12): 8746-52.

2、Verrey F. et al. (2004) CATs and HATs: the SLC7 family of amino acid transporters. Pflugers Arch. 447(5): 532-542.

3、Meier C. et al. (2002) Activation of system L heterodimeric amino acid exchangers by intracellular substrates. EMBO J. 21(4): 580-589.

Background

CD antigen CD98 is also known as 4F2 cell-surface antigen heavy chain (4F2hc), 4F2 heavy chain antigen, Solute carrier family 3 member 2 (SLC3A2), Lymphocyte activation antigen 4F2 large subunit, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family. SLC3A2 constitutes the heavy chain part of HATs and is an essential structure for transporting amino acids, while the light chain part (i.e. LATs) contains catalytic groups that catalyze the transport of amino acids. HATs transport almost all essential amino acids, and different HATs have specific transport functions for different amino acids. Among them, SLC3A2/LAT1 is mainly responsible for transporting most neutral amino acids (such as leucine, isoleucine, methionine, etc.) and aromatic amino acids, while SLC3A2/LAT2 can mediate the transport of all neutral amino acids, and is also the transporter of thyroid hormone. SLC3A2 is widely expressed in a variety of rapidly proliferating cells, and is involved in regulating a variety of important cell activities, playing an important role in cell growth and embryonic development. SLC3A2 has dual functions of regulating amino acid transport and integrin downstream signaling pathways, and studies have found that the extracellular region and the cytoplasmic region of SLC3A2 are two relatively independent functional regions, in which the extracellular region is closely related to amino acid transport, while the cytoplasmic region and transmembrane region are necessary for the interaction with integrins.
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SKU: 73263750516

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