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Description
Human MGAT3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge it at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be tested or stored at -20°C or -80°C. However, repeated freezing and thawing should be avoided. 3. Urine, saliva, sweatOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | β-1,4-Mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase is an enzyme encoded by the MGAT3 gene. Over 100 different glycosyltransferases are believed to be involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the β-linked mannose of the melamine core of an N-linked oligosaccharide, generating a double-stranded GlcNAc residue. Multiple alternatively spliced variants encoding the same protein have been identified. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, urine, saliva, sweat, and other biological fluids |
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4.4 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Big Book
Format: Hardcover
It has everything you want to know. It's a big book. Solid.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 4, 2026
★★★★★ 5
Final Fantasy Ultimania Archive Review
Format: Hardcover
This is a quick review of the book from darkhorses latest production the Final Fantasy Ultimania Archive.
This book coves covers final fantasy 1-6 in various sections. The various sections for all the final fantasy games are listed as follows :
1. Art
2. Final Fantasy (Insert number here) Story
3. Characters
4. World
5. Monsters
6. Extra Content - goes into different sub catagories and varies from final fantasy to final fantasy.
7. Last section is - Final Fantasy (Insert number here) Memories.
I have to say I really like this book a lot, but then again It's a dark horse bool publication of course it's going to be good. The pages are THICKKK. When I thought they were glued together or something by accident but nope, these pages are just really that thick. The hardcover is nice, although best to keep it clean since it's mostly all white.
My favorite section is probably the character section. Not only does it list which character is conected to who in some way as well as the villians but also it shows Yoshitaka Amano complicated and beautiful artwork bellow each of the characters pixilated forms. It really gives you a perspective in how Amanos art work was translated into these sprites. Even though the section on art is nothing special seeing as I own "The Sky The Art Of Final Fantasy Slipcase Edition" which I recommend btw, It is still no less nice to see his work here too. I do think we get to see more of his monster desgins here though, but also the art covers for each variation of the game like from the Famicon to Android to PlayStation. It also shows the different logos that were in other platforms/handhelds or media devices. Really cool stuff here.
The story section is OK, doesn't really go indepth about it though. Nice but overall just an OK section.
The world section is pretty cool, showing the entire map of each game. The final fantasy 1 map is super basic, with some green here, dots of green for bushes, some yellow there, etc. Overall a neat little section.
The monster section does the same thing with the characters section in the sense that it shows you Yoshitaka Amano fantasic drawings along side the pixel forms they take in the games. Incredibly fascinating if I do say so myself.
The extra content section is were you see the technical side of what went to making into these games like the sprite data, the map, color list and so on and so forth. This section varies from final fantasy to final fantasy but it gets really technical about it, really cool.
The final section the memories section is just that, memories of the game with random images and quotes of the game.
Final Verdict - 10 out of 10 I did check in the back of the book for a final section called creators voices which are short interviews with the grand masters of the final fantasy series itself and how they feel about their work. Really nice. Overall this is a well done book by dark horse books, yet another fantastic release guys. I highly recommend buying this book regardless if your a collector or not.
My recommendations? Pre-order Volume 2 of The Final Fantasy Ultimania Archive, it comes out in december but still. As well as Pre-Order any of the upcomimg dark horse books, they are all really good regardless if you played the games or not.
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Reviewed in the United States on July 24, 2018
★★★★★ 5
Great for my collection!
Format: Hardcover, Format: Hardcover
Great for my final fantasy collection!
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Reviewed in the United States on May 26, 2026
★★★★★ 5
A Must-Have for any Final Fantasy Fan
Format: Hardcover
GeminiGeek's Honest Review!
If I had to review this Ultimania Volume 1 with a single word, I'd simply say: "FANTASTIC!". This first volume of the Final Fantasy Ultimania books covers the first six Final Fantasy games, and has an enormous amount of details and content. Each game has it own individual section, complete with images of each character's classic sprites, and even their official Yoshitaka Amano art work as well. Aside from being visually appealing, the book is overflowing with unique story details, character bios, and even an encyclopedia of items and monsters! This volume of the Ultimania series is thick, it is huge, but not to the point of being a pain to carry around or hold. If you enjoy any of the six Final Fantasy games that this volume covers, you'll find something to love in this book, there is just so much information and artwork contained within this tome.
This is a must-have for anyone who enjoys the Final Fantasy series, I can't recommend it enough. As a long-time Final Fantasy fan myself, I love this book, and I can't wait for Volume 2 to come out this fall.
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Reviewed in the United States on July 26, 2018
★★★★★ 5
This is the book I've been waiting for!!!
Format: Hardcover
This book has a ton of great information for fans of the first 6 Final Fantasy games. It includes artwork, sketches, maps, computer renderings, and more! Check out the video flip through of the book that I added. If you are a fan, you won't be disappointed!
Note: many characters, monsters, and locations are shown with Japanese language (Katakana or Hiragana most often - sometimes Kanji) with a Romaji translation (such as Kokaturisu for Cockatrice) and a final English translation. Note that much of this book is based off multiple versions of the games - often the PSP / PS1 versions for 1, 2, 4, 5 and DS for 3. Just a note because translations are different for each version. Also some of the sprites shown in character models are from later releases, not original. But it is certainly forgivable since they often show the sprites for multiple versions.
I am just pawing through it non-stop. I feel like a kid when I got the first Final Fantasy and literally wore out the map/monster chart that was included!
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Reviewed in the United States on July 24, 2018