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Description
Organotial kidney organ class training kitProduct Specification Description Composition: Component name Specification Human kidney cancer organoid medium A 100mL Primary culture buffer B 250mL Human kidney cancer primary tissue digestive juice C 30mL Organoid passage digestive juice D 30mL Tissue preservation solution E 100mL Organoid cryopreservation fluid F 20mL Organoid subculture buffer G 250mL Instructions 1, primary (1) tissue after sampling suggested 2 8 The tissues were stored under C
Product Specification
| Description |
Composition:
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| Instructions |
1, primary (1) tissue after sampling suggested 2-8° The tissues were stored under C conditions and quickly transferred to a clean laboratory for tissue processing and cell separation. The information was photographed and registered. (2) Several culture dishes were prepared and primary culture buffer B, precooled at 4 ° C, was added for later use. (3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm3in volume using ophthalmic scissors or scalpel. (4) The tissues were digested with human renal cancer primary tissue digestion solution C and shaken at 37℃ for 15-25min (the digestion was observed at any time during the digestion process). (5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. (6) The filtrate was filtered by a sieve with a pore size of 100um. After collecting the filtrate, the supernatant was removed after enrichment and centrifugation at 300g for 5min. The supernatant was resuspended by adding primary culture buffer B, and the supernatant was removed after enrichment and centrifugation at 300g for 5min. (7) The cell precipitate contained red blood cells, the supernatant was discarded, 1-2 ml of red blood cell lysate was added for 1-2min, diluted to 10mL, and centrifuged at 300g for 5min. (8) Matrix glue calculation: The collected tissue volume was observed after step 6, and the paving plate was resuspended by adding matrix glue (abs9495) at 25 times the tissue volume. (9) For the example of 24-well cell culture plate, 25uL-30uL tissue matrix glue mixture was dispensed to each well for paving (Note: matrix glue was maintained at 0-4 ° C for operation). (10) The paved culture plate was placed in an incubator at 37 ° C for 10-15min. After the matrix gel solidified, 500-750uL of human renal cancer organoid medium A (returned to room temperature) was added for culture. 2, Organoid subculture (1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube, and allowed to rest for 10-15min at 4 ° C. (every 6-8 Wells are a group) (3) a: When the number of organoids is insufficient or the volume is small: Discard the supernatant by centrifugation at 300g for 5min, add 1mL organoid passage culture buffer G, resuspension and transfer into 1.5mL centrifuge tube, and discard the liquid by centrifugation at 300g for 5min for the fourth step. b: When organoids are large in number or size: Centrifugation at 300g for 5min to discard the supernatant, add 1-2mL organoid passage digestion solution D for 2-4min, add 3 times the volume of organoid passage culture buffer G to terminate digestion, centrifugation at 300g for 5min to discard the supernatant, add an appropriate amount of organoid passage culture buffer G to resuspend and transfer to a 1.5mL centrifuge tube. The supernatant was discarded by centrifugation at 300g for 5min, and the fourth step was carried out. (4) After organoids were collected, they were resuspended by adding matrigel, and 25uL of Matrigel per well was spread in 24-well cell culture plates and placed in an incubator for 10-15min. After the Matrigel had set, 500 to 750uL of human renal cancer organoid medium A was added to each well. 3, organoids were frozen (1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. (4) Add 2mL organoid cryopreservation solution F to every 3 Wells, gently blow and mix, and transfer to cell cryopreservation tubes, 1mL per tube. (5) Make the labeling information, after the program cooling, move to -80° C refrigerator, 48h later, transferred to liquid nitrogen for long-term storage. Or put it into the 4℃ refrigerator for 40min, put it into the -20℃ refrigerator for 2h, move it to the -80℃ refrigerator, and put it into the liquid nitrogen tank for storage after 48h. 4, organoid resuscitation (1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. (2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. (3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted in a short time. (4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended, then transferred to a 1.5mL centrifuge tube and centrifuged at 300g for 5min, and the supernatant was discarded. (5) Add 100ul of Matrigel to each tube of cryopreserved tube and resuspend, 25uL of Matrigel in each well was spread in 24-well cell culture plate, placed in the incubator for 10-15min to gel, and 500-750uL of human renal cancer organoid medium A was added. |
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| Storage Temp. | Stored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details. |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
I love it!
Size: TKL- Tenkeyless, Size: TKL- Tenkeyless
This wrist pad is the right size, approx. 14” wide. It provides support and doesn’t hurt my wrist. I needed a wrist rest that was comfortable and this one is awesome. It’s has some firmness, but it’s not hard, and has some give to it, see video. I also like the fabric, it feels good against your skin and doesn’t collect lint.
The plastic gel pads always seem too hard for me, not providing comfort. Glad to find this wrist pad on Amazon!
It fits well next to a laptop or large iPad (especially if you have the keyboard to go with the iPad). The wrist pad allows me to work in comfort. With my laptop, I use this wrist pad along with a soft mousepad, on the right side of my laptop.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 5, 2025
★★★★★ 5
Great!
Size: Compact
Super soft and very comfortable! It perfectly fits my 75% keyboard. It was almost impossible to find one at this size and I'm glad I did. There's no "overhang" at all and its flush with my keyboard. It stays put and isn't slippery at all and it's just the right height too.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 16, 2026
★★★★★ 5
Great Gel Wrist Pad
Size: Full Size
My husband games with the boys every weekend, and he had finally splurged on a gaming system and new monitors. I bought this for him so he can rest his wrists while using the keyboard. He says the quality is great, length perfectly fits the keyboard, it does feel like it is cooling, nice design, very durable so far, not too high so his wrists sit comfortably for typing. Would purchase again but it looks like we won't need to as it is holding up very well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 6, 2026
★★★★★ 5
Its a buy for me. I love this.
Size: TKL- Tenkeyless
The wrist rest is perfect, it stays cool, it feels great on the wrists, perfect height, good value for money, feels very durable, the shape and smell is perfect.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 8, 2026
★★★★★ 5
Comfortable, Simple, Reliable
Size: Tenkeyless, Pattern Name: Keyboard, Size: Tenkeyless, Pattern Name: Keyboard
This is a phenomenal product, and exactly what I wanted. I used to have wrist pain while gaming; I bought this, and then completely forgot that I used to get wrist pain while gaming.
The foam is firm, but comfortable; it’s very supportive, but also flat and consistent. It’s cool, so my wrist never gets sweaty or hot just from using the wrist rest. Its rubberized bottom prevents it from moving at all while gaming. I’ve used it for about a year, and the foam has no indentation at all; in fact, there’s not noticeable wear of any kind.
My one complaint, that I’ve seen in other reviews as well, is that the fabric skin of the wrist rest isn’t quite fit over the foam: there’s some extra material, and it can wrinkle slightly.
Overall, I’m quite satisfied with the product! It’s completely eliminated my wrist pain, and I’ve gotten a year of use out of it with no problems so far. I’d say that’s more than worth the fair price, and I would buy it again without hesitation!
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Reviewed in the United States on April 17, 2026
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