SKU: 94008934307

Rat IL-10 High Sensitivity ELISA Kit

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Description

Rat IL-10 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti rat IL 10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 10 in the sample binds to the solid phase antibody. After

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, biotin labeled IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.22 pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 94-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

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SKU: 94008934307

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Clint
Los Angeles, US
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iPad case is great
Color: Black
Fits great. Like the stand feature on the back
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mmanderson8
Cuba, US
★★★★★ 5
Exactly as described
Color: Red2
Very sturdy and definitely protects my iPad. Great buy even has a part of the pen. Fits perfectly no issues putting it on and it has layers to ensure that your iPad is protected. Color is exactly as pictured. Easy to use and charges fine with the case on
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Draper, US
★★★★★ 5
Not broken yet.
Color: Light Purple
I bought this for my daughter’s tablet and she has put it through the test. It has been dropped multiple times and the tablets still good. It was easy to put on, looks good, and does the job.
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Amazon Customer
San Leandro, US
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Durable and good case!
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Excellent sturdy case to protect iPad. Would buy again!
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Kelley Mitchell
New York, US
★★★★★ 5
Well made
Color: Blue
It’s great, better than I expected. I love I love the color and the fit is perfect. Highly recommend!
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Reviewed in the United States on May 30, 2026

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