SKU: 42507682884

Human FGF Acidic ELISA Kit

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Description

Human FGF Acidic ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human FGF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.19 pg/ml

Detection Range: 125 - 8000 pg/ml

Recovery Range: 96-113%

Storage Method: 2-8 ℃

Standard Curve

Background:

Fibroblast growth factor acidic (FGF acidic), also known as FGF-1, endothelial cell growth factor (ECGF) and heparin binding growth factor-1 (HBGF-1) are non glycosylated 17-18 kDa polypeptides secreted by a variety of cell types. This molecule is synthesized as an amino acid without obvious signal peptide sequence(AA) protein. Therefore, this ruled out the possibility that it was secreted through the canonical er/ Golgi pathway. However, it seems to have a unique secretion pathway including heat shock protein, phosphatidylserine, synaptotagmin-1. The result of acidic secretion of FGF is that extracellular disulfide bonds connect homodimers, and its covalent bonds may be destroyed by reducing agents to release bioactive FGF acidity. Unlike FGF-2, acidic FGFs are known to have a 5'alternate start site. The acidic FGF of a 60 AA splice variant has been reported to be a full-length molecule consisting of the first 57 amino acids plus three additional C-terminal amino acids. In experiments, it can bind FGF receptors, but will not activate them. Acidic FGF has a nuclear localization sequence consisting of aa residues 21-27. Acidic FGF exhibits 95% AA properties for mice and rats and 92% AA properties for cattle. Cells known to express acidic FGFs include mammary epithelial cells and neurons (motor and sensory), skeletal muscle and smooth muscle cells, renal proximal tubular cells, endothelial cells, macrophages, keratinocytes, fibroblasts. The FGF family has five receptors (FGF R1-R5). The first four receptors are transmembrane tyrosine kinase receptors, and FGF R5 is a member of IgSF without tyrosine kinase domain. Notably, acidic FGFs have been reported to bind receptors 1 to 4, but not FGF R5, with FGFs. Acidic FGFs can activate tyrosine kinase binding or use the signal pathway of ligand receptor internalization to reach organelles and nuclei in cells. Receptor activation is associated with concurrent FGF heparin interactions. Heparin can bind with acidic FGF to inhibit or activate the receptor. When activated, heparin appears to be a central component connecting the two fgf/fgf r complexes. This is an approximation of two FGF r molecules to initiate receptor dimerization and signal transduction. Acidic FGFs also bind heparin on the cell surface, with internalization but no cell activation. Acidic FGF is famous for its mitotic activity on endothelial cells. Activation and internalization of membrane receptors are necessary for complete mitosis. Other cells that proliferate in response to acidic FGF include fibrocytes, muscle cells, hepatocytes, mammary epithelium and fibroblasts.

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SKU: 42507682884

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H. Simon
Houston, US
★★★★★ 5
Great Sheets!
Size: Twin, Color: Oatmeal
Super soft, wash up beautifully, seem well made — very happy! It’s very hard to find 100% cotton t-shirt type sheets and these are really lovely.
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Reviewed in the United States on June 1, 2026
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Chris
Chelsea, US
★★★★★ 3
Weirdly textured
Size: King, Color: Dark Gray
I have bought and loved jersey sheets in the past, that have all been as soft as a well worn cotton t-shirt. These arent soft. They have the stretch of jersey, but not the soft smooth texture that they should have. Even after washing a few times these are one of my least favorite sheets I've ever bought on Amazon.
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Reviewed in the United States on May 30, 2026
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Nyfan1
Charlottesville, US
★★★★★ 5
Soft, Breathable Sheets That Feel Great to Sleep On
Size: Twin, Color: White
This Amazon Basics jersey sheet set was a really good purchase. The cotton material is soft, stretchy, and breathable — it feels like sleeping in a comfortable T‑shirt. The fitted sheet has a nice snug fit that stays in place, and the flat sheet is lightweight without feeling flimsy. The fabric washes well, doesn’t pill, and stays soft after multiple loads. It’s a great everyday sheet set for anyone who likes that cozy, relaxed jersey feel. Overall, comfortable, easy to care for, and great value for the price.
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Reviewed in the United States on May 18, 2026
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Christopher
Lowell, US
★★★★★ 5
The Perfect Sheets To Lie On While Writing Amazon Reviews
Size: Full, Color: Light Gray
As I write this, I am laying on an adjustable full sized bed by Hofish and a 10.5" comfort foam mattress by Classic Brands. I know I sound like I am an Amazon plant, but I swear I am not. I'm not even a stockholder anymore. This is the most awesome bed stylings combination I have had in many, many years. In fact, it is the best I have ever owned and it is beat only by some ritzy resort I stayed at on vacation like 20 years ago, though I admit I was pretty drunk the entire time so my memory might not be the most accurate. But getting back to today... these sheets are great. I washed them twice - I'm not a neat freak, I am just amazingly disorganized and easily distracted while doing laundry, so tonight they went though two wash cycles and one dry. And then I put them on the bed. While these sheets are not deep pocket sheets, they don't need to be with this mattress. There is enough room and border for them to tuck and wrap with the elastic to give a strong grip under the mattress, which is important as the adjustable frame continually put the sheets and mattress though its repeated adjustable calesthenics-type positioning. The other two sheet sets I have used since I got this bed have struggled poorly with this. In the day or so and at least a dozen position changes, these sheets stay in place with no popped corners to exposing the bowl as the head and feet rise. Of course, till will tell if it lasts, but so far they are the best of the batch of sheets in my closet. As for texture, they are awesome. I'm a dude. I don't really know what thread count or knit or whatever really means. But these sheets feel like a well-washed favorite, quality rock & roll concert t-shirt. And not one of these glitter puff stupid new groups, like a real rock band from the 80s that now charges $250 for a concert ticket and $60 for a t-shirt...but that group you saw back then, paid $10 for the shirt and you STILL have it and love to wear it because it is perfectly molded to your body, soft and reliable. The kind of shirt you will wear under your suit at work and just grin not just for mental middle finger to corporate dress codes, but because the t-shirt is just that great of a feeling. Well, that is what these sheets feel like. And now you get to sleep in it. It is that awesome. In LA right now we are having a nasty heat wave, but these sheets don't seem to bake me even with my stuttering AC. The flannel sheets I love seem to trigger the butt sweats as soon as I lay down in this heat, but no such problem with these. So really, I'm absolutely happy with these things. I am going to order more in other colors as soon as Amazon gets them back in stock. Great price, great quality, great comfort, great fit, great deal. It is that simple.
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Reviewed in the United States on September 3, 2017
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Marellen Coley
Los Angeles, US
★★★★★ 5
Soft smooth and wrinkle free!
Size: King, Color: White
The most comfortable sheets I’ve had in years! Soft and don’t wrinkle up in the bed. Some reviews said they are thin but I think they’re perfect! I don’t want super thick sheets as we are hot sleepers. These are the perfect feel. They also stretch very well to fit on my mattress all the way around and I have a thick mattress! My precious sheets were a struggle to get over the corners but these fit perfectly. Would buy 10 times over! Such a good value as well!
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Reviewed in the United States on April 14, 2026

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