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Description
Normal liver organ class training kit Organotial pigProduct Specification Instructions 1, primary (1) The harvested tissues should be placed in precooled (2 8 C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. (2) Prepare several culture dishes and add primary culture buffer B precooled at 4 C for later use. (3) The sampling bottle was disinfected, and the tissue was
Product Specification
| Instructions |
1, primary (1) The harvested tissues should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. (2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. (3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm3in volume using ophthalmic scissors or scalpel. (4) The tissues were digested with porcine normal liver primary tissue digestion solution C and shaken at 37℃ for 15-30min (the digestion was observed at any time during the digestion). (5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. (6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. (7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. (8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). (9) The laid culture plate was placed in an incubator at 37 ° C for 10-15min to become gelation, and porcine normal liver organoid medium A was added for culture (return to room temperature). 2, Organoid subculture (1) The medium was sucked out by pipetting gun, and 1-2mL of 4℃ organoid subculture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. (4) After the organoids were collected, 25uL of matrigel was added to the 24-well cell culture plate, and 500uL of pig normal liver organoid medium A was added to the incubator for 10-15min. 3, organoids were frozen (1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4 ° C organoid passage culture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. (4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. (5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. 4, organoid resuscitation (1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. (2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. (3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. (4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. (5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of porcine normal liver organoid medium A was added. |
| Storage Temp. | Stored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details. |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
Bigger then I thought it would be.
Scent: Graphite Gray_4 Pcs
The only complaint I have is that in the picture it looks like its about the size of a normal bar of soap. In reality though you could fit like 4-5 bars of soap in this thing, it's way bigger then I thought it would be, in fact it's huge. At first I thought this was a negative thing, but after using it a few times I realized that with the insert they give you it's fine and the soap basically stays in place because of the insert.
Quality: Seems to be decent quality
Value for money: Yeah I would say it's a decent value
Roughness: Doesn't seem too rough
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Reviewed in the United States on July 10, 2025
★★★★★ 5
A Winter Essential I Keep Reaching For
Scent: Graphite Gray_4 Pcs
I love these body scrubbers, especially now during the winter season when exfoliating dry skin is a must. The texture is just right and scrubby enough to smooth out rough spots, yet gentle on sensitive areas. I also appreciate how the pouch holds leftover soap bits so nothing goes to waste. The graphite gray color looks sleek in the shower, and the lift pads help the scrubbers dry quickly and stay fresh. They lather beautifully and make my skin feel noticeably softer after each use. This set has quickly become one of my favorite simple self-care upgrades.
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Reviewed in the United States on December 2, 2025
★★★★★ 5
Durability and cleanliness
Scent: Graphite Gray_4 Pcs
I use the soap saver pouch everyday. I honestly don’t use it for what it’s intended for besides cleaning my body. I use body wash instead of soap bars so it defeats the purpose. But this review isn’t about that. It’s more about the durability and feeling of cleanliness after using it. I don’t need to apply a lot of pressure because it is abrasive. So a light rub with body wash does the job. And if you’re to use it on your face like I do, you have to use even LESS pressure because face is MUCH more sensitive. I even wash my hair with it (I’m almost bald) but I have dandruff. I don’t have to use the shampoo anymore after scrubbing my head with this. Anyways I feel the soap savor pouch works very efficiently in getting all the dead skin off my body. I love using this product over a washcloth or those other products. WARNING: Not good to use for sensitive skin. Product is abrasive and will irritate sensitive skin.
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Reviewed in the United States on June 10, 2021
★★★★★ 3
As described—great for exfoliating and saving soap bits
Scent: Graphite Gray_4 Pcs
These myHomeBody soap pockets are exactly as described. They work well for exfoliating in the shower and are especially handy for using up leftover soap bits. The mesh is just rough enough for a good scrub without being too harsh on the skin. The graphite gray color looks nice and doesn’t stain. The included lift pads are a nice bonus to keep bars dry between uses. Great value for a practical 2-pack.
Q&A Section:
Q: Are these gentle enough for daily use?
A: Yes, the mesh exfoliates effectively but is still comfortable on the skin for daily use.
Q: Can you fit a full bar of soap inside?
A: Absolutely—fits full-size bars or small leftover pieces.
Q: What are the lift pads for?
A: They keep your soap elevated and dry between uses to extend its life.
Q: Do the pockets lather well?
A: Yes, they create a good lather and help stretch out your soap use.
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Reviewed in the United States on July 30, 2025
★★★★★ 5
Nice for the price
Scent: Graphite Gray_4 Pcs
I thought they would be really rough when I got them, but they softened up. You get a nice scrub without being too rough.
They are well built and look like they should last.
They lather up well too
Very happy with this purchase
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Reviewed in the United States on July 13, 2025